Sequencing:Article Title: Identification of commensals and molecular components mediating trypsin degradation in the large intestine
Article Snippet: In some earlier experiments SDS-PAGE and PVDF membrane (0.2μm Transfer Membranes Immobilon-PSQ Merck Millipore Ltd.) transfer were performed according to the manufacturer’s [XV PANTERA SYSTEM(DRC)] instructions. iBindTM Western Systems (Thermo Fisher Scienti c) were used for staining throughout the study. .. Antibodies used in this study are as follows: Rabbit anti-mouse PRSS2 (Cosmo Bio Co., Ltd., CPA, Japan, custom-made), Rabbit anti-mouse HSP90 antibody (#4877, Cell Signaling TECHNOLOGY), Rabbit anti-human PRSS2 (LS-B15726, LSBio), Rabbit anti-human PRSS1 (LS-331381, LSBio), Rabbit anti-mouse TMPRSS2 (LS-C373022, LSBio, raised against a sequence at the protease domain), Rabbit anti-6-His Antibody(A190-214A, Bethyl laboratories), Goat anti-mouse IgA alpha-chain (HRP) (ab97235, Abcam), Rat anti-mouse kappa-chain (HRP) (ab99632, Abcam), Rabbit anti-mouse CELA3b (OACD03205, Avivasysbio), Anti-rabbit IgG (HRP-linked Antibody) (#7074, Cell Signaling TECHNOLOGY), Rabbit anti-mouse Reg3b (51153-R005, Sino Biological). .. ChemiLumi One (nacalai tesque) was used for chemiluminescence assay and Molecular imager® ChemiDocTM XRS+ system (BIO-RAD) or iBrightTM FL1500 were used for imaging.
Article Title: Identification of trypsin-degrading commensals in the large intestine
Article Snippet: In some earlier experiments, SDS–PAGE and PVDF membrane (0.2 μm Transfer Membranes Immobilon-P SQ , Merck Millipore) transfer were performed according to the manufacturer’s (XV Pantera System (DRC)) instructions. iBind Western Systems (Thermo Fisher Scientific) were used for staining throughout the study. .. The antibodies used in this study are as follows: rabbit anti-mouse PRSS2 (Cosmo Bio, CPA, Japan, custom-made), rabbit anti-mouse HSP90 antibody (4877, C45G5, Cell Signaling Technology), rabbit anti-human PRSS2 (LS-B15726, LSBio), rabbit anti-human PRSS1 (LS-331381, LSBio), rabbit anti-mouse TMPRSS2 (LS-C373022, LSBio, raised against a sequence at the protease domain), rabbit anti-6-His (A190-214A, Bethyl laboratories, to probe His-tagged recombinant mouse PRSS2 (rmPRSS2) and human PRSS3 (rhPRSS3)), goat anti-mouse IgA alpha-chain (HRP) (ab97235, Abcam), rat anti-mouse kappa-chain (HRP) (ab99632, Abcam), rabbit anti-mouse CELA3b (OACD03205, Avivasysbio), anti-rabbit IgG (HRP-linked antibody) (7074, Cell Signaling Technology), rabbit anti-mouse Reg3β (51153-R005, Sino Biological). .. Rabbit anti-6-His antibodies (A190-214A, Bethyl laboratories) were used to probe rmPRSS2 throughout the study except for the experiment in Fig. , for which rabbit anti-mouse PRSS2 (Cosmo Bio, CPA, custom-made) was used to differentiate rmPRSS2 from recombinant 00502 and 00509 (also His-tagged).
Article Title: Identification of trypsin-degrading commensals in the large intestine.
Article Snippet: In some earlier experiments, SDS–PAGE and PVDF membrane (0.2 μm Transfer Membranes Immobilon-PSQ, Merck Millipore) transfer were performed according to the manufacturer’s (XV Pantera System (DRC)) instructions. iBind Western Systems (Thermo Fisher Scientific) were used for staining throughout the study. .. The antibodies used in this study are as follows: rabbit anti-mouse PRSS2 (Cosmo Bio, CPA, Japan, custom-made), rabbit anti-mouse HSP90 antibody (4877, C45G5, Cell Signaling Technology), rabbit anti-human PRSS2 (LS-B15726, LSBio), rabbit anti-human PRSS1 (LS-331381, LSBio), rabbit anti-mouse TMPRSS2 (LS-C373022, LSBio, raised against a sequence at the protease domain), rabbit anti-6-His (A190-214A, Bethyl laboratories, to probe His-tagged recombinant mouse PRSS2 (rmPRSS2) and human PRSS3 (rhPRSS3)), goat anti-mouse IgA alpha-chain (HRP) (ab97235, Abcam), rat anti-mouse kappa-chain (HRP) (ab99632, Abcam), rabbit anti-mouse CELA3b (OACD03205, Avivasysbio), anti-rabbit IgG (HRP-linked antibody) (7074, Cell Signaling Technology), rabbit anti-mouse Reg3β (51153-R005, Sino Biological). .. Rabbit anti-6-His antibodies (A190-214A, Bethyl laboratories) were used to probe rmPRSS2 throughout the study except for the experiment in Fig. 3j, for which rabbit anti-mouse PRSS2 (Cosmo Bio, CPA, custom-made) was used to differentiate rmPRSS2 from recombinant 00502 and 00509 (also His-tagged).
Recombinant:Article Title: Identification of trypsin-degrading commensals in the large intestine
Article Snippet: In some earlier experiments, SDS–PAGE and PVDF membrane (0.2 μm Transfer Membranes Immobilon-P SQ , Merck Millipore) transfer were performed according to the manufacturer’s (XV Pantera System (DRC)) instructions. iBind Western Systems (Thermo Fisher Scientific) were used for staining throughout the study. .. The antibodies used in this study are as follows: rabbit anti-mouse PRSS2 (Cosmo Bio, CPA, Japan, custom-made), rabbit anti-mouse HSP90 antibody (4877, C45G5, Cell Signaling Technology), rabbit anti-human PRSS2 (LS-B15726, LSBio), rabbit anti-human PRSS1 (LS-331381, LSBio), rabbit anti-mouse TMPRSS2 (LS-C373022, LSBio, raised against a sequence at the protease domain), rabbit anti-6-His (A190-214A, Bethyl laboratories, to probe His-tagged recombinant mouse PRSS2 (rmPRSS2) and human PRSS3 (rhPRSS3)), goat anti-mouse IgA alpha-chain (HRP) (ab97235, Abcam), rat anti-mouse kappa-chain (HRP) (ab99632, Abcam), rabbit anti-mouse CELA3b (OACD03205, Avivasysbio), anti-rabbit IgG (HRP-linked antibody) (7074, Cell Signaling Technology), rabbit anti-mouse Reg3β (51153-R005, Sino Biological). .. Rabbit anti-6-His antibodies (A190-214A, Bethyl laboratories) were used to probe rmPRSS2 throughout the study except for the experiment in Fig. , for which rabbit anti-mouse PRSS2 (Cosmo Bio, CPA, custom-made) was used to differentiate rmPRSS2 from recombinant 00502 and 00509 (also His-tagged).
Article Title: Identification of trypsin-degrading commensals in the large intestine.
Article Snippet: In some earlier experiments, SDS–PAGE and PVDF membrane (0.2 μm Transfer Membranes Immobilon-PSQ, Merck Millipore) transfer were performed according to the manufacturer’s (XV Pantera System (DRC)) instructions. iBind Western Systems (Thermo Fisher Scientific) were used for staining throughout the study. .. The antibodies used in this study are as follows: rabbit anti-mouse PRSS2 (Cosmo Bio, CPA, Japan, custom-made), rabbit anti-mouse HSP90 antibody (4877, C45G5, Cell Signaling Technology), rabbit anti-human PRSS2 (LS-B15726, LSBio), rabbit anti-human PRSS1 (LS-331381, LSBio), rabbit anti-mouse TMPRSS2 (LS-C373022, LSBio, raised against a sequence at the protease domain), rabbit anti-6-His (A190-214A, Bethyl laboratories, to probe His-tagged recombinant mouse PRSS2 (rmPRSS2) and human PRSS3 (rhPRSS3)), goat anti-mouse IgA alpha-chain (HRP) (ab97235, Abcam), rat anti-mouse kappa-chain (HRP) (ab99632, Abcam), rabbit anti-mouse CELA3b (OACD03205, Avivasysbio), anti-rabbit IgG (HRP-linked antibody) (7074, Cell Signaling Technology), rabbit anti-mouse Reg3β (51153-R005, Sino Biological). .. Rabbit anti-6-His antibodies (A190-214A, Bethyl laboratories) were used to probe rmPRSS2 throughout the study except for the experiment in Fig. 3j, for which rabbit anti-mouse PRSS2 (Cosmo Bio, CPA, custom-made) was used to differentiate rmPRSS2 from recombinant 00502 and 00509 (also His-tagged).
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